diff --git a/ArchiSteamFarm/Localization/Strings.Designer.cs b/ArchiSteamFarm/Localization/Strings.Designer.cs
index c5b65d113..5e507ddeb 100644
--- a/ArchiSteamFarm/Localization/Strings.Designer.cs
+++ b/ArchiSteamFarm/Localization/Strings.Designer.cs
@@ -963,6 +963,15 @@ namespace ArchiSteamFarm.Localization {
             }
         }
         
+        /// <summary>
+        /// Wyszukuje zlokalizowany ciąg podobny do ciągu ASF process is already running for this working directory, aborting!.
+        /// </summary>
+        public static string ErrorSingleInstanceRequired {
+            get {
+                return ResourceManager.GetString("ErrorSingleInstanceRequired", resourceCulture);
+            }
+        }
+        
         /// <summary>
         /// Wyszukuje zlokalizowany ciąg podobny do ciągu Could not check latest version!.
         /// </summary>
diff --git a/ArchiSteamFarm/Localization/Strings.resx b/ArchiSteamFarm/Localization/Strings.resx
index 8f9864968..e13eeb3fc 100644
--- a/ArchiSteamFarm/Localization/Strings.resx
+++ b/ArchiSteamFarm/Localization/Strings.resx
@@ -717,4 +717,7 @@ StackTrace:
     <value>Bot has {0} games remaining in its background queue.</value>
     <comment>{0} will be replaced by remaining number of games in BGR's queue</comment>
   </data>
+  <data name="ErrorSingleInstanceRequired" xml:space="preserve">
+    <value>ASF process is already running for this working directory, aborting!</value>
+  </data>
 </root>
\ No newline at end of file
diff --git a/ArchiSteamFarm/NLog/Logging.cs b/ArchiSteamFarm/NLog/Logging.cs
index 4f975c427..2cbdcc333 100644
--- a/ArchiSteamFarm/NLog/Logging.cs
+++ b/ArchiSteamFarm/NLog/Logging.cs
@@ -139,7 +139,7 @@ namespace ArchiSteamFarm.NLog {
 			return !string.IsNullOrEmpty(result) ? result.Trim() : null;
 		}
 
-		internal static void InitCoreLoggers() {
+		internal static void InitCoreLoggers(bool uniqueInstance) {
 			if (LogManager.Configuration != null) {
 				IsUsingCustomConfiguration = true;
 				InitConsoleLoggers();
@@ -156,14 +156,18 @@ namespace ArchiSteamFarm.NLog {
 			config.AddTarget(coloredConsoleTarget);
 			config.LoggingRules.Add(new LoggingRule("*", LogLevel.Debug, coloredConsoleTarget));
 
-			FileTarget fileTarget = new FileTarget("File") {
-				DeleteOldFileOnStartup = true,
-				FileName = SharedInfo.LogFile,
-				Layout = GeneralLayout
-			};
+			if (uniqueInstance) {
+				FileTarget fileTarget = new FileTarget("File") {
+					CleanupFileName = false,
+					ConcurrentWrites = false,
+					DeleteOldFileOnStartup = true,
+					FileName = SharedInfo.LogFile,
+					Layout = GeneralLayout
+				};
 
-			config.AddTarget(fileTarget);
-			config.LoggingRules.Add(new LoggingRule("*", LogLevel.Debug, fileTarget));
+				config.AddTarget(fileTarget);
+				config.LoggingRules.Add(new LoggingRule("*", LogLevel.Debug, fileTarget));
+			}
 
 			LogManager.Configuration = config;
 			InitConsoleLoggers();
diff --git a/ArchiSteamFarm/OS.cs b/ArchiSteamFarm/OS.cs
index 6e0beedfe..31de27ba9 100644
--- a/ArchiSteamFarm/OS.cs
+++ b/ArchiSteamFarm/OS.cs
@@ -22,7 +22,9 @@
 using System;
 using System.IO;
 using System.Runtime.InteropServices;
+using System.Text;
 using System.Text.RegularExpressions;
+using System.Threading;
 using ArchiSteamFarm.Localization;
 using JetBrains.Annotations;
 
@@ -33,6 +35,8 @@ namespace ArchiSteamFarm {
 		[NotNull]
 		internal static string Variant => RuntimeInformation.OSDescription.Trim();
 
+		private static Mutex SingleInstance;
+
 		internal static void Init(bool systemRequired, GlobalConfig.EOptimizationMode optimizationMode) {
 			if (RuntimeInformation.IsOSPlatform(OSPlatform.Windows)) {
 				DisableQuickEditMode();
@@ -60,6 +64,24 @@ namespace ArchiSteamFarm {
 			}
 		}
 
+		internal static bool RegisterProcess() {
+			if (SingleInstance != null) {
+				return false;
+			}
+
+			string uniqueName = "Global\\" + SharedInfo.AssemblyName + "-" + Convert.ToBase64String(Encoding.UTF8.GetBytes(Directory.GetCurrentDirectory()));
+
+			Mutex singleInstance = new Mutex(true, uniqueName, out bool result);
+
+			if (!result) {
+				return false;
+			}
+
+			SingleInstance = singleInstance;
+
+			return true;
+		}
+
 		internal static void UnixSetFileAccessExecutable(string path) {
 			if (string.IsNullOrEmpty(path) || !File.Exists(path)) {
 				ASF.ArchiLogger.LogNullError(nameof(path));
@@ -73,6 +95,16 @@ namespace ArchiSteamFarm {
 			}
 		}
 
+		internal static void UnregisterProcess() {
+			if (SingleInstance == null) {
+				return;
+			}
+
+			SingleInstance.ReleaseMutex();
+			SingleInstance.Dispose();
+			SingleInstance = null;
+		}
+
 		private static void DisableQuickEditMode() {
 			if (Console.IsOutputRedirected) {
 				return;
diff --git a/ArchiSteamFarm/Program.cs b/ArchiSteamFarm/Program.cs
index cda791977..bfe22f533 100644
--- a/ArchiSteamFarm/Program.cs
+++ b/ArchiSteamFarm/Program.cs
@@ -118,7 +118,12 @@ namespace ArchiSteamFarm {
 			Target.Register<HistoryTarget>(HistoryTarget.TargetName);
 			Target.Register<SteamTarget>(SteamTarget.TargetName);
 
-			InitCore(args);
+			if (!await InitCore(args).ConfigureAwait(false)) {
+				await Exit(1).ConfigureAwait(false);
+
+				return;
+			}
+
 			await InitASF(args).ConfigureAwait(false);
 		}
 
@@ -141,7 +146,7 @@ namespace ArchiSteamFarm {
 			await ASF.Init().ConfigureAwait(false);
 		}
 
-		private static void InitCore(IReadOnlyCollection<string> args) {
+		private static async Task<bool> InitCore(IReadOnlyCollection<string> args) {
 			Directory.SetCurrentDirectory(SharedInfo.HomeDirectory);
 
 			// Allow loading configs from source tree if it's a debug build
@@ -166,7 +171,17 @@ namespace ArchiSteamFarm {
 				ParsePreInitArgs(args);
 			}
 
-			Logging.InitCoreLoggers();
+			bool uniqueInstance = OS.RegisterProcess();
+			Logging.InitCoreLoggers(uniqueInstance);
+
+			if (!uniqueInstance) {
+				ASF.ArchiLogger.LogGenericError(Strings.ErrorSingleInstanceRequired);
+				await Task.Delay(5000).ConfigureAwait(false);
+
+				return false;
+			}
+
+			return true;
 		}
 
 		private static async Task InitGlobalConfigAndLanguage() {
@@ -320,6 +335,7 @@ namespace ArchiSteamFarm {
 			// Ensure that IPC is stopped before we finalize shutdown sequence
 			await ArchiKestrel.Stop().ConfigureAwait(false);
 
+			// Stop all the active bots so they can disconnect cleanly
 			if (Bot.Bots?.Count > 0) {
 				// Stop() function can block due to SK2 sockets, don't forget a maximum delay
 				await Task.WhenAny(Utilities.InParallel(Bot.Bots.Values.Select(bot => Task.Run(() => bot.Stop(true)))), Task.Delay(Bot.Bots.Count * WebBrowser.MaxTries * 1000)).ConfigureAwait(false);
@@ -328,8 +344,12 @@ namespace ArchiSteamFarm {
 				await Task.Delay(1000).ConfigureAwait(false);
 			}
 
+			// Flush all the pending writes to log files
 			LogManager.Flush();
 
+			// Unregister the process from single instancing
+			OS.UnregisterProcess();
+
 			return true;
 		}